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Regulation of the Interleukin-5 Receptor α-Subunit on Peripheral Blood Eosinophils from Healthy Subjects by C. HELLMAN; G. HALLDÉN; B. HYLANDER; J. LUNDAHL is a Immunology and Microbiology article available to read on EtoBox.

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## SUMMARY The aim was to study in vitro regulation of the IL-5 receptor α (IL-5Rα) on purified peripheral blood eosinophils from healthy subjects. The IL-5Rα was down-regulated, in a dose-dependent manner, by recombinant IL-5 and GM-CSF, with IL-5 being most potent. This down-regulation was not induced by autocrine release of GM-CSF or IL-5, respectively. Incubation of eosinophils with cell-free peritoneal dialysis fluid (PF) collected from a patient with peritoneal fluid eosinophilia (PFE), induced up-regulation of the proportion of CD69 positive eosinophils, in parallel with down-regulation of the proportion of IL-5Rα positive eosinophils. Experiments with neutralizing antibodies against IL-5 and GM-CSF, revealed that IL-5 was the principal cytokine responsible for the down-regulation of the IL-5Rα. When eosinophils were incubated with PF collected from the same patient in remission or with PF collected from a newly started patient or a patient with bacterial peritonitis, less down-regulation of the IL-5Rα was observed. In conclusion our data indicate that IL-5, as opposed to its proposed action on eosinophil progenitors, down-regulates the IL-5Rα chain on mature eosinophils. We

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Author

C. HELLMAN; G. HALLDÉN; B. HYLANDER; J. LUNDAHL

Publisher

Oxford University Press (OUP)

Published

2003

Language

EN

Field

Immunology and Microbiology (Life Sciences)