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High prevalence of HHV‐6 DNA in peripheral blood mononuclear cells of healthy individuals detected by nested‐PCR by Dr. José I. Cuende; Juan Ruiz; Maria P. Civeira; Jesús Prieto is a Medicine article available to read on EtoBox.

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## Abstract The aim of the study was the evaluation of human herpesvirus‐6 (HHV‐6) infection rate and semiquantification of viremia in healthy people. Healthy blood donors were studied. Human herpesvirus‐6 IgG and IgM antibody titers were measured by indirect immunofluorescence assay. Human herpesvirus‐6 DNA amplification (nested‐PCR) was performed in peripheral blood mononuclear cells (PBMC) and in serum. Seventeen of 50 (34 percent) individuals were positive for IgG anti‐HHV‐6 and the titers ranged from 1:40 to 1:160. None of 30 individuals was positive for IgM anti‐HHV‐6, suggesting no recent infection nor reactivation. Human herpesvirus‐6 DNA was detected by nested‐PCR amplification in peripheral blood mononuclear cells but not in sera. When 1 μg DNA was amplified, HHV‐6 DNA was detected in 8 of 20 individual (40%), but in 18 of 20 (90%) when 5 μg DNA were amplified. It is concluded that HHV‐6 is present in a high proportion of the healthy population but in minimal amounts, and although it can be detected in 1.4 × 10^5^ PBMC, 7 × 10^5^ cells are necessary to detect most cases. No reactivation was observed in healthy people. © 1994 Wiley‐Liss, Inc.

Who reads High prevalence of HHV‐6 DNA in peripheral blood mononuclear cells of healthy individuals detected by nested‐PCR?

Author

Dr. José I. Cuende; Juan Ruiz; Maria P. Civeira; Jesús Prieto

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

1994

Language

EN

Field

Medicine (Health Sciences)