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Can I read Fourth-generation chimeric antigen receptor T cells targeting folate receptor alpha antigen expressed on breast cancer cells for adoptive T cell therapy on EtoBox?
Fourth-generation chimeric antigen receptor T cells targeting folate receptor alpha antigen expressed on breast cancer cells for adoptive T cell therapy by Piriya Luangwattananun; Mutita Junking; Jatuporn Sujjitjoon; Yupanun Wutti-in; Naravat Poungvarin; Chanitra Thuwajit; Pa-thai Yenchitsomanus is a Medicine article available to read on EtoBox.
What is Fourth-generation chimeric antigen receptor T cells targeting folate receptor alpha antigen expressed on breast cancer cells for adoptive T cell therapy about?
## Purpose: Treatment of breast cancer (bc) by standard methods is effective in the early stage, but ineffective in the advanced stage of disease. to develop an adoptive t cell therapy for advanced and severe bc, we generated fourth-generation chimeric antigen receptor (car) t cells targeting folate receptor alpha antigen (frα) expressed on bc cells, and preclinically evaluated their anti-bc activities. ## Methods: The fourth-generation frα-car t cells containing extracellular frα-specific single-chain variable fragment (scfv) and three intracellular costimulatory domains (cd28, 4-1bb, and cd27) linked to cd3ζ were generated using a lentiviral system, and then were evaluated for their anti-bc activities in two-dimensional and three-dimensional (spheroid) cultures. ## Results: When our fourth-generation frα-car t cells were cocultured with frα-expressing mda-mb-231 bc cell line at an effector to target ratio of 20:1, these car t cells specifically lysed 88.7 ± 10.6% of the target cells. interestingly, the cytotoxic lysis of frα-car t cells was more pronounced in target cells with higher surface frα expression. this specific cytotoxicity of the car t cells was not observed when cocul
Who reads Fourth-generation chimeric antigen receptor T cells targeting folate receptor alpha antigen expressed on breast cancer cells for adoptive T cell therapy?
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- Author
- Piriya Luangwattananun; Mutita Junking; Jatuporn Sujjitjoon; Yupanun Wutti-in; Naravat Poungvarin; Chanitra Thuwajit; Pa-thai Yenchitsomanus
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)