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Gas Exchange Is Essential for Bioreactor Cultivation of Tissue Engineered Cartilage by Bojana Obradovic; Rebecca L. Carrier; Gordana Vunjak-Novakovic; Lisa E. Freed is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter × 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (∼40 mmHg) and low pH (∼6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y L/G , of 2.2 mol/mol) while higher oxygen tension (∼80 mmHg) and higher pH (∼7.0) were associated with more aerobic cell metabolism (Y L/G of 1.65-1.79 mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y L/

Who reads Gas Exchange Is Essential for Bioreactor Cultivation of Tissue Engineered Cartilage?

Author

Bojana Obradovic; Rebecca L. Carrier; Gordana Vunjak-Novakovic; Lisa E. Freed

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)