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The document outlines the procedure for preparing and loading samples for SDS-PAGE, including the preparation of stacking and separating gels, as well as the necessary reagents. It also details the process for obtaining a crude bromelain extract from pineapple, including homogenization, filtration, and centrifugation. Finally, it describes the protein precipitation step to concentrate the bromelain extract.

Author

Khanh Thùy

Language

EN