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Effects of Bone Morphogenetic Protein-7 Stimulation on Osteoblasts Cultured on Different Biomaterials by Yahya Açil; Ingo N.G. Springer; Vanessa Broek; Hendrik Terheyden; Søren Jepsen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Effects of Bone Morphogenetic Protein-7 Stimulation on Osteoblasts Cultured on Different Biomaterials about?

The objective of the present study was to investigate the effects of an in vitro stimulation of human osteoblasts by recombinant human bone morphogenetic protein-7 (rhBMP-7) on the collagen types and the quantity of the collagen cross-links synthesized in a three-dimensional culture on various biomaterials for bone replacement. Trabecular bone chips were harvested from human iliac crests, and cell cultures were established at standard conditions. One hundred and fifty nanograms per milliliter of rhBMP-7 was added. For the second passage a cell scraper was used to bring the cells into suspension, and 100 microl osteoblasts (at a density of 3.3 x 10(5)) were transferred onto nine blocks of either Bio-Oss, Tutoplast, or PepGen p-15. Blocks incubated with cells that were not treated with rhBMP-7 served as controls. Cell colonization of the biomaterials was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) after a period of 2, 4, and 6 weeks. Throughout the experiment medium, supernatants were collected and collagen was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Finally, the collagen cross-link residues

Who reads Effects of Bone Morphogenetic Protein-7 Stimulation on Osteoblasts Cultured on Different Biomaterials?

Author

Yahya Açil; Ingo N.G. Springer; Vanessa Broek; Hendrik Terheyden; Søren Jepsen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)