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Can I read Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus on EtoBox?
Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus by Kyle R. Pomraning; Ziyu Dai; Nathalie Munoz; Young-Mo Kim; Yuqian Gao; Shuang Deng; Teresa Lemmon; Marie S. Swita; Jeremy D. Zucker; Joonhoon Kim; Stephen J. Mondo; Ellen Panisko; Meagan C. Burnet; Bobbie-Jo M. Webb-Robertson; Beth Hofstad; Scott E. Baker; Kristin E. Burnum-Johnson; Jon K. Magnuson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus about?
The global regulator LaeA controls secondary metabolism in diverse Aspergillus species. Here we explored its role in regulation of itaconic acid production in __Aspergillus pseudoterreus__. To understand its role in regulating metabolism, we deleted and overexpressed __laeA,__ and assessed the transcriptome, proteome, and secreted metabolome prior to and during initiation of phosphate limitation induced itaconic acid production. We found that secondary metabolite clusters, including the itaconic acid biosynthetic gene cluster, are regulated by __laeA__ and that __laeA__ is required for high yield production of itaconic acid. Overexpression of LaeA improves itaconic acid yield at the expense of biomass by increasing the expression of key biosynthetic pathway enzymes and attenuating the expression of genes involved in phosphate acquisition and scavenging. Increased yield was observed in optimized conditions as well as conditions containing excess nutrients that may be present in inexpensive sugar containing feedstocks such as excess phosphate or complex nutrient sources. This suggests that global regulators of metabolism may be useful targets for engineering metabolic flux that is ro
Who reads Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kyle R. Pomraning; Ziyu Dai; Nathalie Munoz; Young-Mo Kim; Yuqian Gao; Shuang Deng; Teresa Lemmon; Marie S. Swita; Jeremy D. Zucker; Joonhoon Kim; Stephen J. Mondo; Ellen Panisko; Meagan C. Burnet; Bobbie-Jo M. Webb-Robertson; Beth Hofstad; Scott E. Baker; Kristin E. Burnum-Johnson; Jon K. Magnuson
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)