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Determination of Nine Pyrethroid Insecticides by High-performance Liquid Chromatography with Post-column Photoderivatization and Detection Based on Acetonitrile Chemiluminescence by M. Martínez Galera; M.D. Gil García; R. Santiago Valverde is a Chemistry article available to read on EtoBox.

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An HPLC method was developed to determine pyrethroids, including fenpropathrin, ␤-cyfluthrin, -cyhalothrin, deltamethrin, fenvalerate, permethrin, acrinathrin, -fluvalinate, and bifenthrin, by coupling HPLC, post-column irradiation with UV light and chemiluminescence detection of the resulting photoproducts. It is based on the observation that photolyzed pyrethroids take part in a chemiluminescent reaction in presence of K 3 Fe(CN) 6 and NaOH, whose signal increases with the percentage of acetonitrile in the reaction medium. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C 18 , Phenomenex 150 mm × 4.6 mm, 5 m particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactives as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the

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Author

M. Martínez Galera; M.D. Gil García; R. Santiago Valverde

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)