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Microwave-assisted synthesis of [^52^Mn]Mn-porphyrins: Applications in cell and liposome radiolabelling by Peter J. Gawne; Sara M.A. Pinto; Karin M. Nielsen; George P. Keeling; Mariette M. Pereira; Rafael T. M. de Rosales is a Medicine article available to read on EtoBox.
What is Microwave-assisted synthesis of [^52^Mn]Mn-porphyrins: Applications in cell and liposome radiolabelling about?
## Background Manganese porphyrins have several therapeutic/imaging applications, including their use as radioprotectants (in clinical trials) and as paramagnetic MRI contrast agents. The affinity of porphyrins for lipid bilayers also makes them candidates for cell/liposome labelling. We hypothesised that metalation with the positron emission tomography (PET) radionuclide ^52^Mn (__t__~1/2~ = 5.6 d) would allow long-term __in vivo__ biodistribution studies of Mn-porphyrins, as well as a method to label and track cells/liposomes, but methods for fast and efficient radiolabelling are lacking. ## Results Several porphyrins were produced and radiolabelled by addition to neutralised [^52^Mn]MnCl~2~ and heating using a microwave (MW) synthesiser, and compared with non-MW heating. MW radiosynthesis allowed >95 % radiochemical yields (RCY) in just 1 h. Conversely, non-MW heating at 70 °C for 1 h resulted in low RCY (0–25 % RCY) and most porphyrins did not reach radiolabelling completion after 24 h. Formation of the ^52^Mn-complexes were confirmed with radio-HPLC by comparison with their non-radioactive ^55^Mn counterparts. Following this, several [^52^Mn]Mn-porphyrins were used to radiolab
Who reads Microwave-assisted synthesis of [^52^Mn]Mn-porphyrins: Applications in cell and liposome radiolabelling?
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- Author
- Peter J. Gawne; Sara M.A. Pinto; Karin M. Nielsen; George P. Keeling; Mariette M. Pereira; Rafael T. M. de Rosales
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)