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Comparative evaluation of FASTPlaque assay with PCR and other conventional in vitro diagnostic methods for the early detection of pulmonary tuberculosis by Sarman Singh; Taran Prit Saluja; Manjot Kaur; G.C. Khilnani is a Medicine article available to read on EtoBox.

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## Abstract Rapid and accurate diagnosis of symptomatic patients of pulmonary tuberculosis (TB) is highly desirable to minimize the spread of the disease in the society. We, therefore, compared the usefulness of various conventional diagnostic methods, the in‐house polymerase chain reaction (PCR), and the FASTPlaque assay in this study. Laboratory data of 150 patients with clinical diagnosis of pulmonary TB and 50 controls were included in this study. The sputa from all these 200 individuals were subjected to acid‐fast staining, culture on Lowenstein–Jensen (L‐J) slants, automated BACTEC‐MGIT‐960 culture methods, and a mycobacteriophage assay. A mycobacterium genus and __Mycobacterium tuberculosis__ species‐specific PCRs were also done and samples positive on both PCRs were considered as standard for comparison. Of the 5 in vitro diagnostic tests, PCR method was found to be the most rapid, sensitive, and specific, detecting all the 150 cases of pulmonary TB without any false‐positive and negative result. In comparison with PCR the sensitivity of MGIT‐960 was 90%, followed by FASTPlaque assay (76.7%), L‐J culture method (73.3%), and microscopy (60%). The mean detection time for smea

Who reads Comparative evaluation of FASTPlaque assay with PCR and other conventional in vitro diagnostic methods for the early detection of pulmonary tuberculosis?

Author

Sarman Singh; Taran Prit Saluja; Manjot Kaur; G.C. Khilnani

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0887-8013)

Published

2008

Language

EN

Field

Medicine (Health Sciences)