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Spontaneous Activation and Signaling by Overexpressed Epidermal Growth Factor Receptors in Glioblastoma Cells by Christopher Y. Thomas; Michael Chouinard; Michael Cox; Sarah Parsons; Melody Stallings-Mann; Roy Garcia; Richard Jove; Robert Wharen is a Medicine article available to read on EtoBox.

What is Spontaneous Activation and Signaling by Overexpressed Epidermal Growth Factor Receptors in Glioblastoma Cells about?

## Abstract Overexpressed epidermal growth receptor factor receptors (EGFRs) are thought to contribute to the malignant phenotype of human glioblastomas (GBMs), but the mechanism is not well understood. We found that SKMG‐3 cells, a rare GBM cell line that maintains EGFR gene amplification __in vitro__, produced high levels of EGFR protein. The cells also expressed the related receptors HER2/neu and HER4, but not HER3. Immunoblots and tryptic phosphopeptide maps showed that the SKMG‐3 EGFRs were intact and functional and that a subset of these receptors were spontaneously autophosphorylated. EGF treatment stimulated phosphorylation of the EGFRs as well as the downstream effectors Erk, AKT1, stat3 and c‐Cbl. Under minimal growth conditions, the unstimulated SKMG‐3 cells contained constitutively phosphorylated Erk and AKTI but no detectable stat3 DNA‐binding complexes. The EGFR kinase inhibitor PD158780 reduced the constitutive phosphorylation of the receptor and Erk but not that of AKT1. In contrast, inhibition of phosphatidylinositol‐3‐kinase (PI3K) blocked the constitutive phosphorylation of Erk and AKT‐1 but not the EGFR. We conclude that the SKMG‐3 cells represent the subset of

Who reads Spontaneous Activation and Signaling by Overexpressed Epidermal Growth Factor Receptors in Glioblastoma Cells?

Author

Christopher Y. Thomas; Michael Chouinard; Michael Cox; Sarah Parsons; Melody Stallings-Mann; Roy Garcia; Richard Jove; Robert Wharen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2003

Language

EN

Field

Medicine (Health Sciences)