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A Monoclonal Antibody Reactive with a Tumor Antigen That Is Expressed Upon Tumor Progression by P. Rottiers; T. Verfaillie; H. Dooms; M. Desmedt; R. Contreras; W. Fiers; J. Grooten is a Immunology and Microbiology article available to read on EtoBox.

What is A Monoclonal Antibody Reactive with a Tumor Antigen That Is Expressed Upon Tumor Progression about?

introduction: Renal cell carcinoma belongs to the group of human malignancies where spontaneous remission, regression on immunomodulation by cytokines and isolation of tumor reactive T cells in single patients suggest that the immune system might be capable tc mount an effective response to specific tumor antigens. Therefore the AO201, BO801, Cw7 homozygotous renal carcinoma cell line A-498 (ATCC) was used to ldentii MHC class I associated peptides as potential target antigens of cytotoxic T-lymphocytes (CTL). MaterfaIr and Methods: HIA-B and C antigens as well as total HLA class I molecules were isolated by affinity chromatographie using a specific monoclonal antibody (mAb) against HLA-B/C antigens (TH4) and the W8/32. HL mAb against HLA A, B, C. Related peptides were released by subsequent acid elution and further purified by microbore reversed phase-HPLC. Sequencing of various peptides was achieved for the first time by the MALDI-Post-Source Decay (PSD) technique as an analytical tool to obtain sequence information from exceedingly small amounts (100-30 fmoles) of peptides even if present in rather heterogenous samples. HIA A2 positive T2 cells were used to identify HLA A2 deriv

Who reads A Monoclonal Antibody Reactive with a Tumor Antigen That Is Expressed Upon Tumor Progression?

Author

P. Rottiers; T. Verfaillie; H. Dooms; M. Desmedt; R. Contreras; W. Fiers; J. Grooten

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

1997

Language

EN

Field

Immunology and Microbiology (Life Sciences)