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Two-dimensional Capillary Electrophoresis Involving Capillary Isoelectric Focusing and Capillary Zone Electrophoresis by Chun Yang; Lingyi Zhang; Hechun Liu; Weibing Zhang; Yukui Zhang is a Chemistry article available to read on EtoBox.

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Capillary isoelectric focusing (cIEF) and capillary zone electrophoresis (CZE) was on-line hyphenated by a dialysis interface to achieve a 2D capillary electrophoresis (CE) system. The system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). The focused zone in the first dimension (i.e. the cIEF) was driven to the dialysis interface by electroosmotic flow (EOF), besides chemical mobilization from the first anode to the shared cathode. And then in the second dimension (i.e. the CZE), the separated zone was further separated and driven by an inverted EOF, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. Finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional CE implement.

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Author

Chun Yang; Lingyi Zhang; Hechun Liu; Weibing Zhang; Yukui Zhang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)