Can I read Differential Mitogenic Responses of Human Macrovascular and Microvascular Endothelial Cells to Cytokines Underline Their Phenotypic Heterogeneity on EtoBox?

Differential Mitogenic Responses of Human Macrovascular and Microvascular Endothelial Cells to Cytokines Underline Their Phenotypic Heterogeneity by I. Lang; C. Hoffmann; H. Olip; M.-A. Pabst; T. Hahn; G. Dohr; G. Desoye is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Differential Mitogenic Responses of Human Macrovascular and Microvascular Endothelial Cells to Cytokines Underline Their Phenotypic Heterogeneity about?

## Abstract. A variety of growth factors promote the complex multistep process of angiogenesis. The mitogenic activity of vascular endothelial growth factors (VEGFs) and placental growth factors (PlGFs), known as cytokines acting predominantly on endothelial cells, was tested on human umbilical vein endothelial cells (HUVEC) and microvascular endothelial cells (MIEC) and compared with the potency of the universally acting basic fibroblast growth factor (FGF‐2). The cells were seeded at different cell numbers and incubated with various doses of growth factors for a period of 24–72 h in culture medium ± serum. Proliferation was determined by measuring the optical density after staining the cells with the tetrazolium salt WST‐1. VEG__F__~121~ and VEG__F__~165~ increased the number of HUVEC and MIEC at low and high seeding densities various doses and incubation times. The efficiency of FGF‐2 was less pronounced at high seeding densities of the cells under serum‐free conditions. PlGF‐1 and PlGF‐2 stimulated mitogenesis on HUVEC only at low cell numbers and after a short incubation time by 125 ± 3% and 102 ± 5% (__P__ < 0.001), respectively. Longer incubation times with the lower seeding

Who reads Differential Mitogenic Responses of Human Macrovascular and Microvascular Endothelial Cells to Cytokines Underline Their Phenotypic Heterogeneity?

Author

I. Lang; C. Hoffmann; H. Olip; M.-A. Pabst; T. Hahn; G. Dohr; G. Desoye

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7722)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)