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The document outlines the standard procedure for inducing and freezing BL21 cells expressing GST-fusion proteins. Key steps include: 1) Growing BL21 cells overnight in LB medium with antibiotics at 37°C. 2) Inoculating 6L of fresh LB medium with overnight culture and growing at 37°C until OD600 reaches 0.5-1.0. 3) Inducing protein expression by adding IPTG and reducing temperature to 18°C for overnight shaking. 4) Harvesting and centrifuging cells, resuspending in sonication buffer, and storing in 50mL tub

Author

dhashrath

Language

EN