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This document describes a standard procedure for determining the ammonium content in water samples using a spectrophotometric method. The method involves the reaction of ammonium with phenol, sodium nitroprusside, and an oxidizing solution to form a blue compound called indophenol, whose absorbance is measured at 640 nm. Calibration standards of different ammonium concentrations are prepared and a calibration curve is constructed to determine the ammonium concentration in the samples.

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