Can I read Brachyury (T) expression in embryonal carcinoma P19 cells resembles its expression in primitive streak and tail-bud but not that in notochord on EtoBox?

Brachyury (T) expression in embryonal carcinoma P19 cells resembles its expression in primitive streak and tail-bud but not that in notochord by Hirotake Yamaguchi; Tomoaki Niimi; Yasuo Kitagawa; Kiyoshi Miki is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Brachyury (T) expression in embryonal carcinoma P19 cells resembles its expression in primitive streak and tail-bud but not that in notochord about?

__Brachyury (T)__ is involved in mesoderm induction during early mouse development. __T__ expression in embryonal carcinoma P19 cells, which differentiate into mesoderm derivatives __in vitro__, was studied. Endogenous __T__ expression in P19 cells was transiently induced when the cells were allowed to form aggregates. This expression was enhanced by dimethyl sulfoxide. __In situ__ hybridization showed that __T__ expressing cells formed clusters on the aggregates. Transfection of plasmids encoding reporter genes under the control of the upstream region of __T__ showed that the sequence up to – 351 bp can resemble the differentiation‐dependent expression of __T__ in P19 cells. To define the promoter region regulating __T__ expression, transgenic mice carrying __LacZ__ under the control of the upstream region were prepared. The region up to – 351 bp is sufficient to direct the expression in the primitive streak and tail‐bud. The upstream region up to – 2400 bp does not support expression in the notochord. The sequence between – 987 and – 585 bp enhances expression in the primitive streak and tail‐bud. In the tail‐bud where new cells for elongation of the anteroposterior axis were con

Who reads Brachyury (T) expression in embryonal carcinoma P19 cells resembles its expression in primitive streak and tail-bud but not that in notochord?

Author

Hirotake Yamaguchi; Tomoaki Niimi; Yasuo Kitagawa; Kiyoshi Miki

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0012-1592)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)