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Structure Analysis and Performance of a Microbial Community from a Contaminated Aquifer Involved in the Complete Reductive Dechlorination of 1,1,2,2‐Tetrachloroethane to Ethene by Simona Rossetti; Federico Aulenta; Mauro Majone; Gregory Crocetti; Valter Tandoi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structure Analysis and Performance of a Microbial Community from a Contaminated Aquifer Involved in the Complete Reductive Dechlorination of 1,1,2,2‐Tetrachloroethane to Ethene about?
## Abstract An anaerobic microcosm set up with aquifer material from a 1,1,2,2‐tetrachloroethane (TeCA) contaminated site and amended with butyrate showed a complete TeCA dechlorination to ethene. A structure analysis of the microbial community was performed by fluorescence in situ hybridization (FISH) with already available and on purpose designed probes from sequences retrieved through 16S rDNA clone library construction. FISH was chosen as identification tool to evaluate in situ whether the retrieved sequences belong to primary bacteria responsible for the biodegradative reactions. FISH probes identified up to 80% of total bacteria and revealed the absence or the marginal presence of known TeCA degraders and the abundance of two well‐known H~2~‐utilizing halorespiring bacteria, __Sulfurospirillum__ (32.4 ± 8.6% of total bacteria) and __Dehalococcoides__ spp. (14.8 ± 2.8), thereby providing a strong indication of their involvement in the dechlorination processes. These results were supported by the kinetic and thermodynamic analysis which provided indications that hydrogen was the actual electron donor for TeCA dechlorination. The specific probes, developed in this study, for kno
Who reads Structure Analysis and Performance of a Microbial Community from a Contaminated Aquifer Involved in the Complete Reductive Dechlorination of 1,1,2,2‐Tetrachloroethane to Ethene?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Simona Rossetti; Federico Aulenta; Mauro Majone; Gregory Crocetti; Valter Tandoi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)