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Characterization of the Efficiency of a Cell Separation Process by the Extent of Elimination of a Contaminating Cell Type by Walter J. Bruyninckx; Walter H. Vanneste; Peter J.C. Leijh; Ralph Van Furth; Roger E. Vercauteren is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A stepwise approach to the selection of an appropriate technique for a cell separation problem is presented in which the preparative purification of cells is linked to their analytical separation. We have introduced the extent of elimination of a contaminating cell type from the cell type which one chooses to purify, as a separation parameter that characterizes the efficiency of a separation process independently of the relative cell composition of the starting material. In order to compare different separation techniques, a preparative fraction boundary needs to be chosen between the cell types. We defined this boundary in terms of the physical property on which the separation is based such that yield and purity of the isolated cell suspension are optimized simultaneously. With this analytical approach, it was found that a similar elutriation technique separated human and equine mononuclear cells equally well and that the separability of human monocytes and lymphocytes improved when the cells were separated by increasing the limiting sedimentation coefficient value of the elutriation chamber in small increments.

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Author

Walter J. Bruyninckx; Walter H. Vanneste; Peter J.C. Leijh; Ralph Van Furth; Roger E. Vercauteren

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)