Opening book details…
Can I read DNA fingerprinting of Vibrio cholerae and Aeromonas species by pulsed-field minigel electrophoresis on EtoBox?
DNA fingerprinting of Vibrio cholerae and Aeromonas species by pulsed-field minigel electrophoresis by Lilia Lopez-Canovas; Laura Bravo; Jose Herrera; Ana Maria Riveron; Elisa Javer; Axel Sanchez; Rafael Fando; Maria Dolores Noa; Anabel Fernandez is a Engineering article available to read on EtoBox.
What is DNA fingerprinting of Vibrio cholerae and Aeromonas species by pulsed-field minigel electrophoresis about?
## Abstract DNA molecules of __Vibrio cholerae__ and __Aeromonas__ species were prepared by incubating immobilized cells for 4 and 2 h, respectively, with a nonenzymatic solution that contains chemical reagents only (NDSUPlus). This method gave results as reproducible as the enzymatic one that uses proteinase K, and rendered DNA molecules suitable for fingerprinting by mini‐CHEF electrophoresis. As rapid DNA separations at high electric field are achieved in mini‐CHEF chamber with low heat evolution, DNA restriction fragments were separated in 5 h at 10 V/cm in a single resolution window. Then, fragment separations in three resolution windows were done in 15 h. This time is shorter than the one needed by the large CHEF chamber for resolving fragments in a single resolution window. Three windows permitted to include larger numbers of restriction fragments in the calculation of isolate similarities. Both sample preparation and mini‐CHEF electrophoresis may represent an alternative for performing massive epidemiological studies of __V. cholerae__ and __Aeromonas__ species.
Who reads DNA fingerprinting of Vibrio cholerae and Aeromonas species by pulsed-field minigel electrophoresis?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Lilia Lopez-Canovas; Laura Bravo; Jose Herrera; Ana Maria Riveron; Elisa Javer; Axel Sanchez; Rafael Fando; Maria Dolores Noa; Anabel Fernandez
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)
- Published
- 2006
- Language
- EN
- Field
- Engineering (Physical Sciences)