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Type‐II estrogen binding sites in a lymphoblastoid cell line and growth‐inhibitory effect of estrogen, anti‐estrogen and bioflavonoids by Giovanni Scambia; Franco O. Ranelletti; Pierluigi Benedetti Panici; Mauro Piantelli; Carlo Rumi; Francesco Battaglia; Luigi M. Larocca; Amaldo Capelli; Salvatore Mancuso is a Medicine article available to read on EtoBox.
What is Type‐II estrogen binding sites in a lymphoblastoid cell line and growth‐inhibitory effect of estrogen, anti‐estrogen and bioflavonoids about?
Type4 estrogen-binding sites (type-ll EBS) have been demonstrated in the human lymphoblastoid cell line IM-9 using a whole-cell assay with (6,7-'H) estradiol ('H-E,) as tracer. Competition analysis showed that the anti-estrogen tamoxifen and the flavonoids quercetin and rutin competed for (lH)-E, binding to type-It EBS. Growth experiments demonstrated that diethylstilbestrol (DES) tamoxifen (TAM), quercetin and rutin exerted a reversible dose-dependent inhibition of cell proliferation in the range of concentrations between I 0 nM and 10 WM. The relative binding affinity of quercetin, rutin, DES and TAM for type-ll EBS correlated well with their potency as cell growth inhibitors. Moreover, hesperidin, a flavonoid which does not bind to type-ll EBS, was ineffective in inhibiting cell growth. Cell-cycle analysis showed that the growth-inhibitory effect of DES, TAM or quercetin was due to a blocking effect in the G,-G, phases. Our results suggest that high estrogen and anti-estrogen concentrations and flavonoids may regulate IM-9 cell growth through a common mechanism involving a binding interaction with type-ll EBS.
Who reads Type‐II estrogen binding sites in a lymphoblastoid cell line and growth‐inhibitory effect of estrogen, anti‐estrogen and bioflavonoids?
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- Author
- Giovanni Scambia; Franco O. Ranelletti; Pierluigi Benedetti Panici; Mauro Piantelli; Carlo Rumi; Francesco Battaglia; Luigi M. Larocca; Amaldo Capelli; Salvatore Mancuso
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1990
- Language
- EN
- Field
- Medicine (Health Sciences)