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Transcriptional activation through interaction of MBF2 with TFIIA by Feng-Qian Li; Ken-ichi Takemaru; Masahide Goto; Hitoshi Ueda; Hiroshi Handa; Susumu Hirose is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background: Transcriptional activation of the drosopohila melanogaster fushi tarzu gene by ftz-f1 or its silkworm counterpart bmftz-f1 requires two cofactors mbf1 and mbf2 which do not directly bind to dna. mbf1 is a bridging molecule that connects ftz-f1 (or bmftz- f1), mbf2 and tata binding protein tbp. mbf2 is a positive cofactor that activates transcription. ## Results: To elucidate the mechanism of transcriptional activation by mbf2, we isolated a cdna coding for the factor. northern blot analyses showed temporally restricted expression of mbf2 mrna similar to that of bmftz-f1 mrna. the cdna sequence predicts a polypeptide of 10 kda whereas natural mbf2 is a glycoprotein of 22 kda. the deduced amino acid sequence of the factor showed no homology with proteins in the databases. farwestern analyses and glutathione s-transferase interaction assays demonstrated that mbf2 makes a direct contact with the beta-subunit of tfiia. in a hela cell nuclear extract, bacterially expressed recombinant mbf2 activated transcription from various promoters as natural mbf2 did. this activation requires the mbf2-tfiia interaction. when recombinant mbf2 was added to the hela cell nuclear extract

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Author

Feng-Qian Li; Ken-ichi Takemaru; Masahide Goto; Hitoshi Ueda; Hiroshi Handa; Susumu Hirose

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)