Skip to content

Opening book details…

Can I read Insulin Directly Stimulates Mitochondrial Glucose Oxidation in the Heart on EtoBox?

Insulin Directly Stimulates Mitochondrial Glucose Oxidation in the Heart by Karwi, Qutuba G.; Wagg, Cory S.; Altamimi, Tariq R.; Uddin, Golam M.; Ho, Kim L.; Darwesh, Ahmed M.; Seubert, John M.; Lopaschuk, Gary D. is a Medicine article available to read on EtoBox.

What is Insulin Directly Stimulates Mitochondrial Glucose Oxidation in the Heart about?

## Abstract ## Background Glucose oxidation is a major contributor to myocardial energy production and its contribution is orchestrated by insulin. While insulin can increase glucose oxidation indirectly by enhancing glucose uptake and glycolysis, it also directly stimulates mitochondrial glucose oxidation, independent of increasing glucose uptake or glycolysis, through activating mitochondrial pyruvate dehydrogenase (PDH), the rate-limiting enzyme of glucose oxidation. However, how insulin directly stimulates PDH is not known. To determine this, we characterized the impacts of modifying mitochondrial insulin signaling kinases, namely protein kinase B (Akt), protein kinase C-delta (PKC-δ) and glycogen synthase kinase-3 beta (GSK-3β), on the direct insulin stimulation of glucose oxidation. ## Methods We employed an isolated working mouse heart model to measure the effect of insulin on cardiac glycolysis, glucose oxidation and fatty acid oxidation and how that could be affected when mitochondrial Akt, PKC-δ or GSK-3β is disturbed using pharmacological modulators. We also used differential centrifugation to isolate mitochondrial and cytosol fraction to examine the activity of Akt, PKC

Who reads Insulin Directly Stimulates Mitochondrial Glucose Oxidation in the Heart?

It is typically read by researchers, students, and practitioners in Medicine.

Author
Karwi, Qutuba G.; Wagg, Cory S.; Altamimi, Tariq R.; Uddin, Golam M.; Ho, Kim L.; Darwesh, Ahmed M.; Seubert, John M.; Lopaschuk, Gary D.
Publisher
BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1475-2840)
Published
2020
Language
EN
Field
Medicine (Health Sciences)

More by Karwi, Qutuba G.; Wagg, Cory S.; Altamimi, Tariq R.; Uddin, Golam M.; Ho, Kim L.; Darwesh, Ahmed M.; Seubert, John M.; Lopaschuk, Gary D.

Browse all works by Karwi, Qutuba G.; Wagg, Cory S.; Altamimi, Tariq R.; Uddin, Golam M.; Ho, Kim L.; Darwesh, Ahmed M.; Seubert, John M.; Lopaschuk, Gary D.