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HindIII Enzyme Mapping in MCB 120L by Kim is a document available to read on EtoBox.
This document summarizes the results of restriction enzyme mapping of the GAF-4 gene sequence. [1] HindIII was found to cut the GAF-4 sequence at 341 bp. [2] Digesting the PCR product with HindIII produced two bands of approximately 349 bp and 195 bp as expected. [3] Agarose gel analysis verified that the correct PCR product and restriction fragments were obtained, confirming the identity of the amplified Nostoc GAF-4 gene sequence.
- Author
- Kim
- Language
- EN