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RB1CC1 activates the promoter and expression of RB1 in human cancer by Kaichiro Ikebuchi; Tokuhiro Chano; Yasuko Ochi; Hitosuke Tameno; Taketoshi Shimada; Yasuo Hisa; Hidetoshi Okabe is a Medicine article available to read on EtoBox.

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## Abstract __RB1__‐inducible coiled‐coil 1 (RB1CC1, also known as FIP200) is a tumor suppressor implicated in the regulation of RB1 (retinoblastoma 1) expression. However, the molecular mechanism of RB1 regulation by RB1CC1 has not been elucidated. Here, we demonstrate that nuclear RB1CC1 binds to the __RB1__ promoter using chromatin immunoprecipitation assays with anti‐RB1CC1 antibody. Luciferase assays with __RB1__ promoter reporter plasmids revealed that RB1CC1 activated the __RB1__ promoter through the 201 bp upstream GC‐rich region (from the initiation ATG). Electrophoretic mobility shift assay and Western blot analysis supported RB1CC1 binding to the GC‐rich region of the __RB1__ promoter. In addition, the C‐terminus of RB1CC1 was required for nuclear localization and subsequent __RB1__ promoter activation. Furthermore, the expression levels of RB1CC1 and RB1 significantly correlated with __in vivo__ breast cancer tissues as determined by immunohistochemical analysis. These data indicate that nuclear RB1CC1 directly activates the __RB1__ promoter to enhance RB1 expression in cancer cells. Evaluation of RB1CC1 in various types of human cancer tissues is expected to provide us

Who reads RB1CC1 activates the promoter and expression of RB1 in human cancer?

Author

Kaichiro Ikebuchi; Tokuhiro Chano; Yasuko Ochi; Hitosuke Tameno; Taketoshi Shimada; Yasuo Hisa; Hidetoshi Okabe

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2009

Language

EN

Field

Medicine (Health Sciences)