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Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil‐activating properties of adsorbed host proteins—Toward a mucosal mimic by Tomas Sandberg; Marjam Karlsson Ott; Jan Carlsson; Adam Feiler; Karin D. Caldwell is a Engineering article available to read on EtoBox.

## Abstract In continuation of our recent fractionation and characterization study on mucins of bovine salivary (BSM), porcine gastric (PGM), and human salivary (MG1) origin, this study evaluates the effect of mucin precoating on the conformation and neutrophil‐activating properties of host proteins adsorbed to a polyethylene terephthalate‐based model biomaterial. Microscopy combined with assays for the neutrophil releases of reactive oxygen species and human neutrophil lipocalin showed that mucin precoating greatly reduced the strong immune‐response normally induced by adsorbed immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), respectively. A similar finding was made for the proinflammatory fibrinogen. Although the total uptakes of these proteins depended on the mucin surface concentration, a detailed composite analysis suggested the fraction of surface‐exposed protein to be a stronger determinant of coating performance. The unexpectedly low neutrophil activation showed by composites containing near‐monolayer concentrations of exposed IgG and sIgA, respectively, suggested that these act synergistically with mucin on the surface. In support of this hypothesis, quartz cr

Author

Tomas Sandberg; Marjam Karlsson Ott; Jan Carlsson; Adam Feiler; Karin D. Caldwell

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)