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Inhibition of Nipah Virus by Defective Interfering Particles by Welch, Stephen R; Tilston, Natasha L; Lo, Michael K; Whitmer, Shannon L M; Harmon, Jessica R; Scholte, Florine E M; Spengler, Jessica R; Duprex, W Paul; Nichol, Stuart T; Spiropoulou, Christina F is a Medicine article available to read on EtoBox.
What is Inhibition of Nipah Virus by Defective Interfering Particles about?
## Abstract The error-prone nature of RNA-dependent RNA polymerases drives the diversity of RNA virus populations. Arising within this diversity is a subset of defective viral genomes that retain replication competency, termed defective interfering (DI) genomes. These defects are caused by aberrant viral polymerase reinitiation on the same viral RNA template (deletion DI species) or the nascent RNA strand (copyback DI species). DI genomes have previously been shown to alter the dynamics of a viral population by interfering with normal virus replication and/or by stimulating the innate immune response. In this study, we investigated the ability of artificially produced DI genomes to inhibit Nipah virus (NiV), a highly pathogenic biosafety level 4 paramyxovirus. High multiplicity of infection passaging of both NiV clinical isolates and recombinant NiV in Vero cells generated an extensive DI population from which individual DIs were identified using next-generation sequencing techniques. Assays were established to generate and purify both naturally occurring and in silico-designed DIs as fully encapsidated, infectious virus-like particles termed defective interfering particles (DIPs).
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- Author
- Welch, Stephen R; Tilston, Natasha L; Lo, Michael K; Whitmer, Shannon L M; Harmon, Jessica R; Scholte, Florine E M; Spengler, Jessica R; Duprex, W Paul; Nichol, Stuart T; Spiropoulou, Christina F
- Publisher
- University of Chicago Press; Oxford University Press; Oxford University Press (OUP) (ISSN 0022-1899)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)