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Inhibition of Calmodulin-dependent Enzymes in Rat Brain by Hexachlorocyclohexane by B. S. Basavarajappa; Bharathi P. Salimath is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Abstract Ca^2+^ homeostasis is one of the major regulatory mechanisms operating in the nervous system, with calmodulin translating the Ca^2+^ message into cellular response. To check if hexachlorocyclohexane (HCH) acts as a calmodulin antagonist in the nervous system of rats, the in‐vitro effect of HCH on calmodulin‐dependent Ca^2+^‐ATPase and cAMP‐phosphodiesterase (PDE) in rat brain has been studied. In the membrane fraction from rat brain, a basal activity of Ca^2+^‐ATPase was obtained in the absence of Ca^2+^. Inclusion of Ca^2+^ (1 mM) increased the enzyme activity by 70%. Further, addition of fluphenazine, a potent calmodulin antagonist, inhibited the Ca^2+^‐dependent enzyme activity (IC~50~ = 85 μM), demonstrating the calmodulin dependence of the enzyme activity. The Ca^2+^‐ and calmodulin‐dependent Ca^2+^‐ATPase was inhibited by HCH in a dose‐dependent manner (IC~50~ = 80–90 μM). Ca^2+^‐ and calmodulin‐dependent cAMP‐PDE from the cytosolic fraction of rat brain was inhibited by HCH (340 μM) by 79%. Addition of excess calmodulin reversed the inhibitory effects of HCH or fluphenazine on Ca^2+^‐ATPase and cAMP‐PDE, suggesting their direct interaction with calmodulin. By flu

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Author

B. S. Basavarajappa; Bharathi P. Salimath

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-498X)

Published

1990

Language

EN

ISBN

9782780290406

Field

Agricultural and Biological Sciences (Life Sciences)