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Involvement of angiotensin II receptor subtypes during testicular development in rats by H. KANEHARA; K. SONG; K. HIRAI; H. UEDA; N. SHIOTA; H. AZUMA; Y. KATSUOKA; H. MIYAZAKI; M. MIYAZAKI is a Medicine article available to read on EtoBox.

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Expression of testicular angiotensin II (AT2) receptors in Sprague–Dawley rats at various stages of development (1 and 5 days, 2, 3, 4 and 7 weeks postnatal) were studied by in vitro autoradiography and Northern blot analysis. The receptors were labelled with ^125^I‐[Sar^1^, Ile^8^]AT2 and differentiated into two subtypes according to their susceptibility to AT1 (losartan, 5 μM) or AT2 (PD123319, 5 μM) antagonists. Total AT2 receptor binding in the testis was highest at 1 day of age (8.12 ± 0.35 fmol/mg protein, mean ± secEM, __n__ = 8) and decreased gradually thereafter (5 days: 6.9 ± 0.41, 2 weeks: 2.85 ± 0.10, 3 weeks: 1.64 ± 0.19, 4 weeks: 0.76 ± 0.09, 6 weeks: 0.77 ± 0.09 fmol/mg protein, __n__ = 8–11). AT2 receptor binding was strikingly abundant in 1‐day‐old rat testis (6.98 ± 0.34 fmol/mg protein), while considerably less AT1 receptor binding (1.46 ± 0.19 fmol/mg protein) was observed. The relative amounts of each subtype did not change for the first 3 weeks but the 4‐week‐old rat testis contained almost exclusively AT1 receptors (0.63 ± 0.05 fmol/mg protein). Northern blot analysis showed that mRNA expression of both AT1 and AT2 types decreased with age. Microscopic emulsi

Who reads Involvement of angiotensin II receptor subtypes during testicular development in rats?

Author

H. KANEHARA; K. SONG; K. HIRAI; H. UEDA; N. SHIOTA; H. AZUMA; Y. KATSUOKA; H. MIYAZAKI; M. MIYAZAKI

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0105-6263)

Published

1998

Language

EN

Field

Medicine (Health Sciences)