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Mosquito ( Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes by Laurence Duchesne; Jean‐François Hubert; Jean‐Marc Verbavatz; Daniel Thomas; Patricia V. Pietrantonio is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mosquito ( Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes about?
Previous results showed that mRNA encoding a putative aquaporin (AQP) (GenBank accession number AF218314) is present in the tracheolar cells associated with female __Aedes aegypti__ Malpighian tubules. In this study, immunohistochemistry detected the protein, __Aea__AQP, also in tracheolar cells, suggesting its involvement in water movement in the respiratory system. When expressed in __Xenopus__ oocytes, __Aea__AQP increased the osmotic water permeability from 15 × 10^−6^ to 150 × 10^−6^ m·s^−1^, which was inhibited by mercury ions. No permeability to glycerol or other solute was observed. __Aea__AQP expressed in oocytes was solubilized as a homotetramer in nondenaturing detergent as deduced from velocity centrifugation on density gradients. Phylogenetic analysis of MIP (major intrinsic protein) family sequences shows that __Aea__AQP clusters with other native orthogonal array forming proteins. Specific orthogonal arrays were detected by freeze‐fracture analysis of __Aea__AQP oocyte membranes. We conclude that, in tracheolar cells of __A. aegypti__, __Aea__AQP is probably a highly water‐permeable homotetrameric MIP which natively can form 2D crystals__.__
Who reads Mosquito ( Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Laurence Duchesne; Jean‐François Hubert; Jean‐Marc Verbavatz; Daniel Thomas; Patricia V. Pietrantonio
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)