Can I read Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma on EtoBox?

Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma by Aminuddin, Amnani; Ng, Pei Yuen; Leong, Chee-Onn; Chua, Eng Wee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma about?

Cisplatin is the first-line chemotherapeutic agent for the treatment of oral squamous cell carcinoma (OSCC). However, the intrinsic or acquired resistance against cisplatin remains a major obstacle to treatment efficacy in OSCC. Recently, mitochondrial DNA (mtDNA) alterations have been reported in a variety of cancers. However, the role of mtDNA alterations in OSCC has not been comprehensively studied. In this study, we evaluated the correlation between mtDNA alterations (mtDNA content, point mutations, large-scale deletions, and methylation status) and cisplatin sensitivity using two OSCC cell lines, namely SAS and H103, and stem cell-like tumour spheres derived from SAS. By microarray analysis, we found that the tumour spheres profited from aberrant lipid and glucose metabolism and became resistant to cisplatin. By qPCR analysis, we found that the cells with less mtDNA were less responsive to cisplatin (H103 and the tumour spheres). Based on the findings, we theorised that the metabolic changes in the tumour spheres probably resulted in mtDNA depletion, as the cells suppressed mitochondrial respiration and switched to an alternative mode of energy production, __i.e__. glycolysis.

Who reads Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma?

Author

Aminuddin, Amnani; Ng, Pei Yuen; Leong, Chee-Onn; Chua, Eng Wee

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)