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Active poly‐GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model by Qihui Zhou; Nikola Mareljic; Meike Michaelsen; Samira Parhizkar; Steffanie Heindl; Brigitte Nuscher; Daniel Farny; Mareike Czuppa; Carina Schludi; Alexander Graf; Stefan Krebs; Helmut Blum; Regina Feederle; Stefan Roth; Christian Haass; Thomas Arzberger; Arthur Liesz; Dieter Edbauer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Active poly‐GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model about?
The C9orf72 repeat expansion is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and/or frontotemporal dementia (FTD). Non-canonical translation of the expanded repeat results in abundant poly-GA inclusion pathology throughout the CNS. (GA) 149 -CFP expression in mice triggers motor deficits and neuroinflammation. Since poly-GA is transmitted between cells, we investigated the therapeutic potential of anti-GA antibodies by vaccinating (GA) 149 -CFP mice. To overcome poor immunogenicity, we compared the antibody response of multivalent ovalbumin-(GA) 10 conjugates and pre-aggregated carrier-free (GA) 15 . Only ovalbumin-(GA) 10 immunization induced a strong anti-GA response. The resulting antisera detected poly-GA aggregates in cell culture and patient tissue. Ovalbumin-(GA) 10 immunization largely rescued the motor function in (GA) 149 -CFP transgenic mice and reduced poly-GA inclusions. Transcriptome analysis showed less neuroinflammation in ovalbumin-(GA) 10 -immunized poly-GA mice, which was corroborated by semiquantitative and morphological analysis of microglia/ macrophages. Moreover, cytoplasmic TDP-43 mislocalization and levels of the neurofilament light
Who reads Active poly‐GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Qihui Zhou; Nikola Mareljic; Meike Michaelsen; Samira Parhizkar; Steffanie Heindl; Brigitte Nuscher; Daniel Farny; Mareike Czuppa; Carina Schludi; Alexander Graf; Stefan Krebs; Helmut Blum; Regina Feederle; Stefan Roth; Christian Haass; Thomas Arzberger; Arthur Liesz; Dieter Edbauer
- Publisher
- John Wiley and Sons; EMBO; John Wiley & Sons Ltd.; Chichester: Wiley Europe (ISSN 1757-4676)
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)