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Real-time PCR and high-resolution melt analysis methods for detection of pathogenic species of Brucella by Faramarz Masjedian Jazi; Reza Mirnejad; Vahhab Piranfar; Noor Amir Mozafari; Taghi Zahraei Salehi; Mahmoud Khormali; Mansour Sedighi; Gholam Reza Irajian is a Medicine article available to read on EtoBox.
What is Real-time PCR and high-resolution melt analysis methods for detection of pathogenic species of Brucella about?
## Abstract ## Background: It is of great importance to quickly and accurately detect __Brucella abortus__ and __Brucella melitensis__ from clinical and non-clinical samples because of their high prevalence and high risk in causing brucellosis, a life-threating infectious disease affecting both humans and animals. ## Methods: The current study describes a new method for the detection of brucellosis in clinical samples using real-time polymerase chain reaction (PCR) and high-resolution melt (HRM) curve analysis. This study was conducted on 70 human and 55 animal isolates with more than 1/80 serum antibody titers. Additionally, the accuracy and specificity of the methods were compared. ## Results: The mean range [cycles threshold±standard deviation (C~T~±SD)] for the amplified samples was 15.39–25.15 by real-time PCR. The melting peak range (°C) ±SD for __B. abortus__ and __B. melitensis__ was 90.10±0.4 and 89.70±0.4, respectively, and 10 was reported on peak height. ## Conclusions: The results of HRM analysis can be used for species differentiation and bacterial genotyping according to nucleotide polymorphism. This molecular method could help in diagnosing __Brucella__ quickly and p
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- Author
- Faramarz Masjedian Jazi; Reza Mirnejad; Vahhab Piranfar; Noor Amir Mozafari; Taghi Zahraei Salehi; Mahmoud Khormali; Mansour Sedighi; Gholam Reza Irajian
- Publisher
- Walter de Gruyter GmbH
- Published
- 2017
- Language
- EN
- Field
- Medicine (Health Sciences)