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Selection of reference genes for quantitative RT-PCR studies in striped dolphin ( Stenella coeruleoalba ) skin biopsies by Giacomo Spinsanti; Cristina Panti; Elisa Lazzeri; Letizia Marsili; Silvia Casini; Francesco Frati; Cristina Maria Fossi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Selection of reference genes for quantitative RT-PCR studies in striped dolphin ( Stenella coeruleoalba ) skin biopsies about?
## Background Odontocete cetaceans occupy the top position of the marine food-web and are particularly sensitive to the bioaccumulation of lipophilic contaminants. The effects of environmental pollution on these species are highly debated and various ecotoxicological studies have addressed the impact of xenobiotic compounds on marine mammals, raising conservational concerns. Despite its sensitivity, quantitative real-time PCR (qRT-PCR) has never been used to quantify gene induction caused by exposure of cetaceans to contaminants. A limitation for the application of qRT-PCR is the need for appropriate reference genes which allow the correct quantification of gene expression. A systematic evaluation of potential reference genes in cetacean skin biopsies is presented, in order to validate future qRT-PCR studies aiming at using the expression of selected genes as non-lethal biomarkers. ## Results Ten commonly used housekeeping genes (HKGs) were partially sequenced in the striped dolphin (__Stenella coeruleoalba__) and, for each gene, PCR primer pairs were specifically designed and tested in qRT-PCR assays. The expression of these potential control genes was examined in 30 striped dolph
Who reads Selection of reference genes for quantitative RT-PCR studies in striped dolphin ( Stenella coeruleoalba ) skin biopsies?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Giacomo Spinsanti; Cristina Panti; Elisa Lazzeri; Letizia Marsili; Silvia Casini; Francesco Frati; Cristina Maria Fossi
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)