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The phosphotransferase system (PTS) of Streptomyces coelicolor : Identification and biochemical analysis of a histidine phosphocarrier protein HPr encoded by the gene ptsH by Stephan Parche; Roland Schmid; Fritz Titgemeyer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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HPr, the histidine‐containing phosphocarrier protein of the bacterial phosphotransferase system (PTS) controls sugar uptake and carbon utilization in low‐GC Gram‐positive bacteria and in Gram‐negative bacteria. We have purified HPr from __Streptomyces coelicolor__ cell extracts. The N‐terminal sequence matched the product of an __S. coelicolor orf__, designated __ptsH__, sequenced as part of the __S. coelicolor__ genome sequencing project. The __ptsH__ gene appears to form a monocistronic operon. Determination of the evolutionary relationship revealed that __S. coelicolor__ HPr is equally distant to all known HPr and HPr‐like proteins. The presumptive phosphorylation site around histidine 15 is perfectly conserved while a second possible phosphorylation site at serine 47 is not well‐conserved. HPr was overproduced in __Escherichia coli__ in its native form and as a histidine‐tagged fusion protein. Histidine‐tagged HPr was purified to homogeneity. HPr was phosphorylated by its own enzyme I (EI) and heterologously phosphorylated by EI of __Bacillus subtilis__ and __Staphylococcus aureus__, respectively. This phospho__enol__pyruvate‐dependent phosphorylation was absent in an HPr mutan

Who reads The phosphotransferase system (PTS) of Streptomyces coelicolor : Identification and biochemical analysis of a histidine phosphocarrier protein HPr encoded by the gene ptsH?

Author

Stephan Parche; Roland Schmid; Fritz Titgemeyer

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Physical Sciences)