Can I read Determination of Caffeine and Its Metabolites by Micellar Electrokinetic Capillary Electrophoresis on EtoBox?
Determination of Caffeine and Its Metabolites by Micellar Electrokinetic Capillary Electrophoresis by Yeping Zhao; Craig E. Lunte is a Chemistry article available to read on EtoBox.
What is Determination of Caffeine and Its Metabolites by Micellar Electrokinetic Capillary Electrophoresis about?
The determination of caffeine and its analogues is important for a wide variety of analyses and is performed in an assortment of matrices ranging from food to clinical samples. While reversed-phase HPLC has become the standard analysis protocol in most laboratories, capillary electrophoresis has the advantages of higher separation efficiency and shorter separation time. The micellar capillary electrophoresis (MECC) separation of caffeine and its metabolites, theobromine, paraxanthine, theophylline and 1,3,7-trimethyluric acid was investigated using sodium dodecyl sulphate (SDS) as the micellar phase. The effects of pH, micelle concentration, buffer concentration, ionic strength, buffer salts, applied voltage and injection time were studied to select the optimum conditions for the determination of caffeine and its four analogues in drugs, foods and body fluids. Caffeine and its three analogues were resolved within 120 s with detection limits less than ! /xg/ml. Samples could be analyzed utilizing direct injection with satisfactory resolution and reproducibility.
Who reads Determination of Caffeine and Its Metabolites by Micellar Electrokinetic Capillary Electrophoresis?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yeping Zhao; Craig E. Lunte
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)