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L‐type Ca^2+^ channels and purinergic P2X2 cation channels participate in calcium‐tyrosine kinase‐mediated PC12 growth cone arrest by Benno Schindelholz; Bernhard F. X. Reber is a Neuroscience article available to read on EtoBox.

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## Abstract During development and regeneration of the nervous system, growth cones of the various nerve cells navigate and direct neurite elongation by detecting and responding to cues in the environment. To investigate changes in growth cone behaviour due to calcium influx we used nerve growth factor (NGF)‐induced growth cones of PC12 (rat pheochromocytoma cells) cells as a model. High external concentrations of potassium and ATP depress growth cone motility, induce club‐shaped growth cones and reduce filopodia length and the number and relative F‐actin contents of single growth cones (r.a.c.), respectively. The cellular responses are mediated by a sustained increase in the intracellular free Ca^2+^ concentrations ([Ca^2+^]~i~) as monitored by calcium‐sensitive fluorescent dyes and confocal microfluorimetry. The responses are not detectable in the presence of the protein tyrosine kinase inhibitor genistein. Immunocytochemistry revealed an increased level of tyrosine‐phosphorylated proteins in cell bodies and growth cones but not in cell nuclei. Paxillin, a cytoskeleton‐associated protein located in neurites and growth cones, was detected among the phosphotyrosine proteins. The su

Who reads L‐type Ca^2+^ channels and purinergic P2X2 cation channels participate in calcium‐tyrosine kinase‐mediated PC12 growth cone arrest?

Author

Benno Schindelholz; Bernhard F. X. Reber

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)

Published

2000

Language

EN

Field

Neuroscience (Life Sciences)