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Can I read Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2 on EtoBox?
Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2 by Wenlin Li; HongYan Zhou; Ramzey Abujarour; Saiyong Zhu; Jin Young Joo; Tongxiang Lin; Ergeng Hao; Hans R. Schöler; Alberto Hayek; Sheng Ding is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2 about?
## Abstract Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named tranylcypromine), an inhibitor of lysine-specific demethylase 1, CHIR99021 can cause the reprogramming of human primary keratinocyte transduced with the two factors, Oct4 and Klf4. To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. Our studies suggest that the GSK-3 inhibitor might have a general application to replace transcription factors in both mouse and human reprogramming. Disclosure of potential conflicts of interest is found at the end of this article.
Who reads Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Wenlin Li; HongYan Zhou; Ramzey Abujarour; Saiyong Zhu; Jin Young Joo; Tongxiang Lin; Ergeng Hao; Hans R. Schöler; Alberto Hayek; Sheng Ding
- Publisher
- Oxford University Press (OUP)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)