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Nuclear transplantation in Bombina orientalis and utilization of the Pale mutation as a nuclear marker by Mark S. Ellinger; John T. Carlson is a Environmental Science article available to read on EtoBox.

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## Abstract The discoglossid frog, __Bombina orientalis__, displays many characteristics that make it desirable as a laboratory animal. We report here the first successful transplantation of embryonic nuclei into enucleated eggs of this organism. The participation of the grafted nucleus in the development of the transplant embryos was confirmed with the use of a genetic marker, the __Pale__ mutation. Frogs homozygous for this recessive gene are much lighter in appearance than the wild‐type animals. The use of this trait in appropriate experiments demonstrated that nuclear tansplantation in B. __orientalis__ provides embryos whose nuclei may be reliably interpreted as being derived from the injected nucleus.

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Author

Mark S. Ellinger; John T. Carlson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)

Published

1978

Language

EN

Field

Environmental Science (Life Sciences)