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A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis : purification and characterization by Hua‐ping Li; Zheng Hu; Jiang‐lan Yuan; Han‐dong Fan; Wei Chen; Shi‐jia Wang; Shan‐shan Zheng; Zhong‐liang Zheng; Guo‐lin Zou is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A novel serine protease with fibrinolytic activity named CSP was purified from the culture supernatant of the fungus __Cordyceps sinensis__, a kind of Chinese herbal medicine. Analysis of the purified enzyme by SDS‐PAGE indicated that CSP was a single polypeptide chain with an apparent molecular weight of 31 kDa, and __N__‐terminal sequencing revealed that the first ten amino acid residues of the enzyme were Ala‐Leu‐Ala‐Thr‐Gln‐His‐Gly‐Ala‐Pro‐Trp‐. When casein was used as a substrate, the proteolytic activity of CSP reached its maximum at pH 7.0 and 40 °C. The effect of chemical agents on the enzyme activity indicated that CSP is a serine protease with a free cysteine residue near the active site. It hydrolysed fibrinogen, fibrin and casein with a high efficiency, while hydrolysing bovine serum albumin (BSA) and human serum albumin (HSA) to a lesser extent. CSP was found to be a plasmin‐like protease, but not a plasminogen activator, and it preferentially cleaved the A__α__ chain of fibrinogen and the __α__‐chain of fibrin. Therefore, the extracellular protein CSP may represent a potential new therapeutic agent for the treatment of thrombosis. Copyright © 2007 John Wil

Who reads A novel extracellular protease with fibrinolytic activity from the culture supernatant of Cordyceps sinensis : purification and characterization?

Author

Hua‐ping Li; Zheng Hu; Jiang‐lan Yuan; Han‐dong Fan; Wei Chen; Shi‐jia Wang; Shan‐shan Zheng; Zhong‐liang Zheng; Guo‐lin Zou

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-418X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)