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Binding and fluorescence study on interaction of human serum albumin (HSA) with cetylpyridinium chloride (CPC) by Abdol-Khalegh Bordbar; Asghar Taheri-Kafrani is a Materials Science article available to read on EtoBox.

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Human serum albumin (HSA) is frequently used in biophysical and biochemical studies since it has a well-known primary structure and it has been associated with the binding of many different categories of small molecules. In the present study, results are presented for the binding of cetylpyridinium chloride (CPC) with HSA at various pH and 25 degrees C, as monitored using ion selective membrane electrodes and fluorescence spectroscopy of intrinsic tryptophan. The obtained binding isotherms were analyzed on basis of binding capacity concept and Hill plot in order to determine the Hill parameters of binding sets. The system behaved as a system with two sets of binding sites in all studied situations. The results represent a positive cooperative behavior and the essential role of hydrophobic interactions in both binding sets. The intrinsic binding affinity of second binding set have a similar values and trends at acidic and neutral pHs, that represents the similar unfolded structure at these pHs. CPC quenched the fluorescence arising from Trp group incorporated to HSA. A biphasic behavior was observed in quenching process that confirmed the results of binding study correspond to the e

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Author

Abdol-Khalegh Bordbar; Asghar Taheri-Kafrani

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)

Published

2007

Language

EN

Field

Materials Science (Physical Sciences)