Can I read Improved Direct Electron Transfer and Electrocatalytic Activity of Horseradish Peroxidase Immobilized on Gemini Surfactant–polyvinyl Alcohol Composite Film on EtoBox?

Improved Direct Electron Transfer and Electrocatalytic Activity of Horseradish Peroxidase Immobilized on Gemini Surfactant–polyvinyl Alcohol Composite Film by Lihong Liu; Faqiong Zhao; Liqin Liu; Jing Li; Baizhao Zeng is a Materials Science article available to read on EtoBox.

What is Improved Direct Electron Transfer and Electrocatalytic Activity of Horseradish Peroxidase Immobilized on Gemini Surfactant–polyvinyl Alcohol Composite Film about?

The voltammetric and electrocatalytic behavior of horseradish peroxidase (HRP) immobilized on a cationic gemini surfactant (i.e. C 12 H 25 N(CH 3 ) 2 -C 12 H 24 -N(CH 3 ) 2 C 12 H 25 Br 2 , C 12 -C 12 -C 12 )-polyvinyl alcohol (PVA) composite film-coated glassy carbon electrode (GCE) has been studied. It is found that on the novel composite film HRP presents excellent electroactivity and can exhibit a pair of well-defined voltammetric peaks in 0.10 M pH 7.0 phosphate buffer solution (PBS). The immobilized HRP also presents good bioelectrocatalytic activity, and it can catalyze the reduction of oxygen (O 2 ), hydrogen peroxide (H 2 O 2 ), nitrite ion (NO 2 -) and trichloroacetic acid (TCA). For H 2 O 2 the catalytic current is linear to its concentration in the range of 0.195-97.5 M, and the detection limit is down to 6.5 × 10 -8 M. The response shows Michaelis-Menten feature and the apparent Michaelis-Menten constant is estimated to be 110.5 M. Similarly, the electrode can sense NO 2 -and TCA. In addition, it is observed that the spacer group of gemini surfactant affects the electroactivity of HRP significantly. A spacer group with higher flexibility and hydrophility is favorable t

Who reads Improved Direct Electron Transfer and Electrocatalytic Activity of Horseradish Peroxidase Immobilized on Gemini Surfactant–polyvinyl Alcohol Composite Film?

Author

Lihong Liu; Faqiong Zhao; Liqin Liu; Jing Li; Baizhao Zeng

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)

Published

2009

Language

EN

Field

Materials Science (Physical Sciences)