About this Chemistry article
Fractionation of Apple Procyanidins According to Their Degree of Polymerization by Normal-phase High-performance Liquid Chromatography by Akio Yanagida; Tomomasa Kanda; Tomoya Takahashi; Ayako Kamimura; Takako Hamazono; Shinkichi Honda is a Chemistry article available to read on EtoBox.
A new method was developed for the fractionation of procyanidin oligomers according to their degree of polymerization. Monomeric flavan-3-ols and low molecular mass procyanidins were selectively extracted from the lyophilized powder of apple condensed tannins (ACTs) by methyl acetate extraction. Sequentially, the separation of each oligomer from dimer to pentamer in this extract was carried out by normal-phase high-performance liquid chromatography using a silica-beads packed column. The best separation was achieved with a mobile phase system containing hexane; (1) hexane-methanol-ethyl acetate, (2) hexane-acetone. These sequential treatments can be easily adapted to large-scale fractionation.
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Akio Yanagida; Tomomasa Kanda; Tomoya Takahashi; Ayako Kamimura; Takako Hamazono; Shinkichi Honda
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2000
- Language
- EN
- Field
- Chemistry (Physical Sciences)