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Permeabilization of transformed mouse fibroblasts by 3?-O-(4-benzoyl)benzoyl adenosine 5?-triphosphate and the desensitization of the process by Fernando A. Gonzalez; Dr. Ahmed H. Ahmed; Kevin D. Lustig; Laurie Erb; Gary A. Weisman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

A photoreactive analogue of ATP, 3'-0-(4-benzoyl)benzoyl adenosine 5'triphosphate (BzATP) altered the plasma membrane permeability of transformed 3T6 mouse fibroblasts to normally impermeant molecules as previously reported for ATP, but at lower concentrations. BzATP-induced permeabilization was modulated by pH, temperature, and the ionic composition of the medium similar to the permeabilizing effects of ATP. Conditions known to enhance ATP-induced permeabilization, such as treatment with the mitochondria1 uncoupler carbonyl cyanide-m-chlorophenylhydrazone (CCCP) or the Ca\*+-calmodulin antagonist trifluoperazine also enhanced BzATP-induced permeabilization. Conditions in- hibitory to ATP-induced permeabilization, including chloride replacement or treatment with furosemide or dithiothreitol (DTT), inhibited permeabilization induced by BzATP. The ionic strength of the medium modulated the responsiveness of the cells to ATP and BzATP; a decrease in the ionic strength below isotonicity increased the sensitivity of the cells to the nucleotides, whereas an increase in ionic strength above isotonicity inhibited permeabilization. Prolonged exposure to ATP under non-permeabilizing conditio

Author

Fernando A. Gonzalez; Dr. Ahmed H. Ahmed; Kevin D. Lustig; Laurie Erb; Gary A. Weisman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)