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Electroosmotic Flow Suppressing Additives for Capillary Zone Electrophoresis in a Hydrodynamically Closed Separation System by D Kaniansky; M Masár; J Bielčı́ková is a Chemistry article available to read on EtoBox.
Electroosmotic flow in a hydrodynamically closed capillary zone electrophoresis (CZE) separation compartment must be minimized to achieve high efficiency CZE separations. A group of eight potential electroosmotic flow suppressors was investigated in this context for the separations in fluorinated ethylene-propylene capillary tubes. The suppressors included water soluble methylhydroxyethyl derivatives of cellulose, polyvinylalcohol, polyvinylpyrrolidones and polyethyleneglycols of different molecular masses and Triton X-100. Methylhydroxyethylcellulose derivatives and polyvinylalcohol were found to provide the highest separation efficiencies for a group of model anions when the electroosmotic flow suppressors were used as the carrier electrolyte additives. Using a methylhydroxyethylcellulose coated separation compartment very significant improvements in the separation efficiencies were achieved for polyvinylpyrrolidones and polyethyleneglycols applied in the carrier electrolyte solutions. For example, polyvinylpyrrolidone K 90 applied in this way gave for some of the model analytes the plate height values approaching those estimated in the calculations as theoretical limits for our
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- Author
- D Kaniansky; M Masár; J Bielčı́ková
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)