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What is High-Throughput Nextera Library Prep about?
Lamble et al. developed an improved workflow for high-throughput library preparation using the Nextera system, addressing limitations such as the need for pure DNA templates and accurate quantification. Their modified protocol incorporates a bead-based normalization step, significantly reduces costs, and allows for increased multiplexing capabilities, enabling the simultaneous sequencing of up to 9216 samples. The study demonstrates the robustness of this new method across various genomes with different GC
- Author
- sugitmama
- Language
- EN