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Synthesis of biocompatible Fe3O4 and MnO2 nanoparticles for enhanced tuberization in potato (Solanum tuberosum L.) by Neha Joshi; Abhishek Pathak; Devanshi Chandel Upadhyaya; Suresh Babu Naidu Krishna; Chandrama Prakash Upadhyay is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Iron oxide (Fe 3 O 4 ) and manganese dioxide (MnO 2 ) Nanoparticles (NPs) were synthesized via green synthesis approach using beetroot (Beta vulgaris) leaf extract and evaluated as nanofertilizer for studies of In-vitro microtuberization of potato. Successfully biogenesis of NPs were demonstrated through UV-visible spectroscopy, FTIR, XRD, SEM-EDX, and TEM analysis. In-vitro micortuberization analysis, single nodal explants of potato were placed on media (Murashige-Skoog plant growth medium devoid of original Fe and Mn salt) added with different concentrations of metal oxide-NPs, and physiological, biochemical and molecular changes were observed via using standard methods. The interaction of the NPs with the nodal explants significantly induced early tuber induction and tuber growth upon application of Fe 3 O 4 NPs (4.0 mg L -1 ) and MnO 2 NPs (1.0 mg L -1 ) in comparison to untreated potato tissues. Molecular analysis of potato tissues revealed enhanced expression of primary tuber inducing genes viz. Calcium-Dependent Protein Kinases (StCDPK), Calmodulin (StCaM1), and Lipoxygenase (StLOX) enzyme activity show a positive correlation of tuber induction with added NPs. Further elemen

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Author
Neha Joshi; Abhishek Pathak; Devanshi Chandel Upadhyaya; Suresh Babu Naidu Krishna; Chandrama Prakash Upadhyay
Publisher
Elsevier BV
Published
2022
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)