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Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S. mutans biofilm by M. Zhu; S. Takenaka; M. Sato; E. Hoshino is a Dentistry article available to read on EtoBox.
What is Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S. mutans biofilm about?
The present study investigated whether extracellular polysaccharides inhibit reaction between __Streptococcus mutans__ and its specific immunoglobulin G (IgG) and penetration of the IgG through __S. mutans__ biofilm. The planktonic organisms with or without extracellular polysaccharides were prepared, incubated with rabbit IgG against whole cell of __S. mutans__ and fluorescein isothiocyanate (FITC)‐conjugated goat affinity purified antibody to rabbit IgG. Biofilms with or without extracellular polysaccharides were formed on cover glasses and incubated with rabbit IgG against __S. mutans__ and FITC‐conjugated goat antibody to rabbit IgG. Then, biofilms were stained with propidium iodide. The amount of specific IgG binding on __S. mutans__ was determined by FITC intensity with a fluorescence microplate reader. The penetration of IgG through biofilms was determined by confocal laser scanning microscopy. The results showed that the fluorescence intensity of FITC in planktonic organisms with extracellular polysaccharides was similar to that in planktonic organisms without extracellular polysaccharides, indicating that extracellular polysaccharides did not inhibit the reaction between _
Who reads Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S. mutans biofilm?
It is typically read by researchers, students, and practitioners in Dentistry.
- Author
- M. Zhu; S. Takenaka; M. Sato; E. Hoshino
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0902-0055)
- Published
- 2001
- Language
- EN
- Field
- Dentistry (Health Sciences)