Can I read RNA interference and gene editing on EtoBox?

RNA interference and gene editing by Robert E. Farrell Jr is a book available to read on EtoBox.

What is RNA interference and gene editing about?

RNA interference (RNAi) is a naturally occurring mechanism for gene silencing. It is triggered by double-stranded RNA (dsRNA). The trigger can occur naturally, as in the case of cellular infection by a dsRNA virus, or by the intentional introduction of dsRNA to induce user-directed degradation of the cognate transcript(s). RNAi is fundamentally a two-step process. The first step involves the master enzyme in the RNAi process, a type III endoribonuclease aptly named Dicer. This enzyme is involved in the ATP-dependent digestion of longer dsRNA into 19–21bp siRNA molecules with characteristic 3′ dinucleotide overhangs on both strands. In the second step, siRNA, regardless of the source, becomes part of a multicomponent RNA-induced silencing complex. The siRNA and miRNA transcript control pathways show considerable overlap. The overall RNAi efficiency in an experiment is also a function of the abundance and the stability of both the target mRNA and the encoded protein, and the precise region of the mRNA targeted for scission. Clustered regular interspersed short palindromic repeats (CRISPR) is a natural defense mechanism that evolved in prokaryotes to protect themselves against viral D

Author

Robert E. Farrell Jr

Publisher

Elsevier Science & Technology; Academic Press

Published

2023

Language

EN

ISBN

9780323902212

Subjects

Science, Medical, Biology

Updated

2026-03-24