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Proteomic analysis of Medicago truncatula root plastids by Zeina Daher; Ghislaine Recorbet; Benoît Valot; Frank Robert; Thierry Balliau; Sophie Potin; Benoît Schoefs; Eliane Dumas-Gaudot is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Proteomic analysis of Medicago truncatula root plastids about?
## Abstract Despite the recognized importance of non‐photosynthetic plastids in a wide array of plant processes, the root plastid proteome of soil‐grown plants still remains to be explored. In this study, we used a protocol allowing the isolation of __Medicago truncatula__ root plastids with sufficient protein recovery and purity for their subsequent in‐depth analysis by nanoscale capillary LC‐MS/MS. Besides providing the first picture of a root plastid proteome, the results obtained highlighted the identification of 266 protein candidates whose functional distribution mainly resembled that of wheat endosperm amyloplasts and tobacco proplastids together with displaying major differences to those reported for chloroplasts. Most of the identified proteins have a role in nucleic acid‐related processes (16%), carbohydrate (15%) and nitrogen/sulphur (12%) metabolisms together with stress response mechanisms (10%). It is noteworthy that BLAST searches performed against the proteins reported in different plastidomes allowed detecting 30 putative root plastid proteins for which homologues were previously unsuspected as plastid‐located, most of them displaying a common putative role in part
Who reads Proteomic analysis of Medicago truncatula root plastids?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Zeina Daher; Ghislaine Recorbet; Benoît Valot; Frank Robert; Thierry Balliau; Sophie Potin; Benoît Schoefs; Eliane Dumas-Gaudot
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)